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Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

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Identification of C. auris clade 5 isolates using claID.

Aswathy Narayanan¹, Pavitra Selvakumar^2,3, Rahul Siddharthan^2,3

¹Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore,560064, India.

Medical Mycology

|February 28, 2024

Summary

This summary is machine-generated.

This study identifies a new clade (clade 5) of the multidrug-resistant fungus Candida auris, crucial for tracking its global spread. The findings aid in understanding and predicting the behavior of emerging Candida auris clades.

Keywords:

clade-specific sequences clade-typing diagnostic PCR new clade

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Area of Science:

Medical Mycology
Infectious Diseases
Genomics

Background:

Candida auris is a multidrug-resistant fungus causing global health concerns.
Its variants are classified into clades based on geographical origin.
Tracking these clades is vital for understanding spread and predicting antifungal resistance.

Purpose of the Study:

To expand the clade identification method for Candida auris.
To identify and characterize a newly emerged clade, designated clade 5.
To develop specific primers for clade 5 detection.

Main Methods:

Utilized colony-Polymerase Chain Reaction (PCR) for identification.
Employed whole genome alignments to identify clade-specific sequences.
Designed clade-specific primers through in silico analysis for clade 5.

Main Results:

Successfully identified clade 5 of Candida auris.
Clade 5 was isolated in Iran.
Developed and validated specific primers for clade 5 detection.

Conclusions:

The expanded clade identification method enhances surveillance of Candida auris.
Identification of clade 5 provides crucial data for global tracking and outbreak management.
In silico primer design is effective for rapid identification of emerging fungal clades.