Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation.

Nature methods·2025
Same author

Far-red fluorescent genetically encoded calcium ion indicators.

Nature communications·2025
Same author

Near-infrared fluorogenic RNA for in vivo imaging and sensing.

Nature communications·2025
Same author

Fluorogenic CRISPR for genomic DNA imaging.

Nature communications·2024
Same author

Co-crystal structures of the fluorogenic aptamer Beetroot show that close homology may not predict similar RNA architecture.

Nature communications·2023
Same author

Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based Ca<sup>2+</sup> Biosensor.

International journal of molecular sciences·2021

Related Experiment Video

Updated: May 8, 2026

Time-lapse Imaging of Mitosis After siRNA Transfection
08:21

Time-lapse Imaging of Mitosis After siRNA Transfection

Published on: June 6, 2010

17.1K

Recent advances in methods for live-cell RNA imaging.

Tien G Pham1, Jiahui Wu1

  • 1Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. jiahuiwu@umass.edu.

Nanoscale
|February 28, 2024
PubMed
Summary

Live-cell RNA imaging allows scientists to observe RNA localization and dynamics in real-time. This review focuses on methods for visualizing RNA in living mammalian cells at single-molecule resolution.

More Related Videos

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

7.5K

Related Experiment Videos

Last Updated: May 8, 2026

Time-lapse Imaging of Mitosis After siRNA Transfection
08:21

Time-lapse Imaging of Mitosis After siRNA Transfection

Published on: June 6, 2010

17.1K
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

7.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Ribonucleic acid (RNA) is essential for numerous biological processes, including X chromosome inactivation, genome stability, and embryonic development.
  • Understanding RNA's role requires visualizing its localization and dynamics within living cells.
  • Current imaging techniques are crucial for gaining insights into these fundamental cellular functions.

Purpose of the Study:

  • To provide a comprehensive overview of current live-cell RNA imaging techniques.
  • To highlight methods specifically applicable to visualizing RNA in living mammalian cells.
  • To focus on techniques achieving single-molecule resolution for detailed observation.

Main Methods:

  • Review of existing literature on live-cell RNA imaging methodologies.
  • Focus on techniques enabling visualization of RNA molecules within their native cellular environment.
  • Emphasis on methods providing high spatial and temporal resolution, including single-molecule detection.

Main Results:

  • Identification and categorization of various live-cell RNA imaging approaches.
  • Discussion of the advantages and limitations of different techniques for mammalian cells.
  • Highlighting the progress towards achieving single-molecule resolution in live-cell RNA imaging.

Conclusions:

  • Live-cell RNA imaging is a powerful tool for studying RNA biology.
  • Advancements in imaging technology are enabling unprecedented insights into RNA localization and dynamics.
  • Future research directions include further improving resolution and applicability to complex biological systems.