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mRNA Stability and Gene Expression

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Related Experiment Video

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Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
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Replicative aging in yeast involves dynamic intron retention patterns associated with mRNA processing/export and

Jesús Gómez-Montalvo1, Alvaro de Obeso Fernández Del Valle1, Luis Fernando De la Cruz Gutiérrez1

  • 1Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., México.

Microbial Cell (Graz, Austria)
|February 28, 2024
PubMed
Summary

This study reveals intron retention, a form of alternative splicing, as a key factor in yeast aging. Altered intron retention impacts mRNA processing and protein ubiquitination, offering new insights into aging mechanisms.

Keywords:
Saccharomyces cerevisiaeintron retentionmRNA exportmRNA processingreplicative agingtranscription regulationubiquitination

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A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
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Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

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Area of Science:

  • Molecular Biology
  • Genetics
  • Aging Research

Background:

  • Saccharomyces cerevisiae (baker's yeast) is a model organism for studying aging mechanisms.
  • Known aging mechanisms include genomic instability, oxidative stress, and mitochondrial dysfunction.
  • Transcriptional regulation in yeast aging remains less understood.

Purpose of the Study:

  • To investigate alternative splicing, specifically intron retention, during replicative aging in S. cerevisiae.
  • To identify genes and cellular processes affected by altered intron retention during aging.

Main Methods:

  • Utilized the IRFinder algorithm on existing RNA-seq data.
  • Analyzed intron retention patterns in Saccharomyces cerevisiae during replicative aging.

Main Results:

  • Identified 44 differentially retained introns in 43 genes during yeast replicative aging.
  • Observed no significant changes in overall transcript levels for genes with altered intron retention.
  • Linked altered intron retention to mRNA processing/export in early/middle-aged yeast and protein ubiquitination in older cells.

Conclusions:

  • Uncovered intron retention as a novel layer in the transcriptional regulation of yeast aging.
  • Expanded knowledge on alternative splicing occurrences in baker's yeast.
  • Provided insights into conserved aging mechanisms through yeast model studies.