Related Experiment Video
Updated: Jul 30, 2026

11:27
Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
Reliability of thermal transitions for characterizing DNA from micro-organisms
Abstract:
Data from thermal transitions were evaluated for their usefulness in detecting similarities in the deoxyribonucleic acid (DNA) of micro-organisms. Levels of reproducibility were determined for thermal transition analysis; methods of purification of DNA and the solvents, pH, and temperature intervals used during thermal transitions did not greatly affect the reproducibility of the values obtained. The shape of the thermal transition curve is a stable property of a given kind of DNA, and this property can be described quantitatively and with satisfactory precision.
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