Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...

Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Regio- and enantioselective oxidation by CYP108N12: substrate scope and specificity.

Organic & biomolecular chemistry·2026

Same author

Aerobic soil bacteria adapt to hypoxia by hybridizing fermentation with carbon storage.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Examining selection dynamics and limitations in multi-round protein selection of high diversity libraries.

Protein engineering, design & selection : PEDS·2026

Same author

Investigating the P450-Mediated Cross-Linking of the Atypical GPA Complestatin Using Alternate Peptide Probes.

The Journal of organic chemistry·2026

Same author

Monocyte Migration Emerges from a Divergent Chemokine Signaling Network.

bioRxiv : the preprint server for biology·2026

Same author

P450 Cyptide Synthase AscB Catalyzes Fused Cyclophane at the YxWxH Motif on the Precursor Peptides.

Organic letters·2026

Same journal

Organophosphine-Promoted Decarbynylative Hydrocarbenylation of the Carbon-Carbon Triple Bond.

Organic letters·2026

Same journal

Total Syntheses of BE-54238A and -B.

Organic letters·2026

Same journal

Visible Light-Induced <i>N</i>-Phenylbenzo[<i>c</i>]phenothiazine-Catalyzed α-C(sp<sup>3</sup>)-H Phosphonylation of Secondary Amines via Intramolecular 1,5-HAT.

Organic letters·2026

Same journal

Cobalt-Stabilized Propargylic Oxocarbenium Ions Enable Direct and Asymmetric Nickel(II) Catalyzed Aldol-Like Reactions.

Organic letters·2026

Same journal

Photoinduced Regioselective Sulfonylation/Cyclization of <i>N</i>-Cinnamylenamides toward Sulfonylated Tetrahydropyridines via Catalytic Electron Donor-Acceptor Complexes.

Organic letters·2026

Same journal

Amine-Enabled Electron Donor-Acceptor Complex Catalysis for Cyclopropanation.

Organic letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

An Engineered Biarylitide Cross-Linking P450 from RiPP Biosynthesis Generates Alternative Cyclic Peptides.

Maxine Treisman^1,2, Laura Coe³, Yongwei Zhao^1,2

¹Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.

Organic Letters

|February 28, 2024

Summary

This summary is machine-generated.

Engineered cytochrome P450 enzymes can now create new cyclic tripeptides. This research expands the use of these biocatalysts for producing peptides with specific cross-linking patterns.

More Related Videos

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

Related Experiment Videos

Last Updated: Jul 2, 2025

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

Area of Science:

Biocatalysis
Enzyme Engineering
Peptide Chemistry

Background:

Cytochrome P450 enzymes catalyze cross-linking reactions in ribosomally encoded peptides (RiPPs).
This P450-mediated RiPP cross-linking is a growing field with significant biocatalytic potential.
Existing methods primarily focus on specific cross-linking motifs.

Purpose of the Study:

To engineer the active site of the biarylitide cross-linking enzyme P450_Blt.
To expand the substrate scope of P450-mediated peptide cross-linking.
To demonstrate the production of novel cyclic tripeptides with controlled cross-linking.

Main Methods:

Site-directed mutagenesis of the P450_Blt enzyme's active site.
In vitro assays to assess cross-linking activity on synthetic peptide substrates.
Analysis of peptide products using mass spectrometry and other biochemical techniques.

Main Results:

Successfully engineered P450_Blt to form His-X-Tyr cross-linked peptides.
Demonstrated the formation of Tyr-X-Tyr cross-linked peptides using the engineered enzyme.
Achieved controlled cross-linking states in the resulting cyclic tripeptides.

Conclusions:

Active site engineering of P450_Blt provides a versatile platform for generating diverse cyclic tripeptides.
This approach broadens the utility of cytochrome P450 enzymes in biocatalysis and peptide synthesis.
The findings pave the way for novel peptide-based therapeutics and materials.