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Characterization of a cDNA clone encoding murine mitogen-regulated protein: regulation of mRNA levels in mortal and
Abstract:
Mitogen-regulated protein (MRP) is secreted by certain immortal murine cell lines (Swiss 3T3, BNL) stimulated with serum or particular growth factors. We have identified a cDNA clone that encodes part of the protein and have confirmed that MRP is closely related to, if not identical to, the prolactin-related protein designated proliferin. MRP is not produced by primary mouse embryo fibroblasts to nearly the same extent as it is produced by many immortal or transformed lines. Control of expression of this protein by growth factors is achieved both by regulating the extent of transcription and by regulating the processing of the protein.
Insights
Mitogen-regulated protein (MRP) is secreted by immortal cells stimulated by growth factors. This protein is closely related to proliferin, with expression controlled by transcription and processing.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Mitogen-regulated protein (MRP) is secreted by specific immortal murine cell lines.
- Its secretion is induced by serum or growth factors.
Purpose of the Study:
- To identify the cDNA clone encoding MRP.
- To determine the relationship between MRP and other known proteins.
- To understand the regulation of MRP expression.
Main Methods:
- cDNA cloning and sequencing.
- Protein expression analysis in cell lines.
- Analysis of gene transcription and protein processing.
Main Results:
- A cDNA clone encoding part of MRP was identified.
- MRP is closely related, possibly identical, to proliferin.
- Primary mouse embryo fibroblasts produce significantly less MRP than immortal or transformed cell lines.
- Growth factors regulate MRP expression at both transcriptional and post-transcriptional (processing) levels.
Conclusions:
- MRP is a growth factor-regulated protein closely related to proliferin.
- Immortal and transformed cells exhibit distinct regulation of MRP expression compared to primary cells.
- Growth factor control of MRP involves complex transcriptional and processing mechanisms.