Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

754.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
Synthetic Biology02:55

Synthetic Biology

4.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.7K
DNA Isolation01:24

DNA Isolation

39.0K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.0K
Next-generation Sequencing03:00

Next-generation Sequencing

88.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.8K
DNA Base Pairing02:27

DNA Base Pairing

27.4K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
27.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The transcriptional gradient in negative-strand RNA viruses suggests a common RNA transcription mechanism.

PLoS computational biology·2026
Same author

Within-Host Environmental Heterogeneity Is Associated With Phenotypic but Not Genomic Diversity in Wolbachia Endosymbionts.

Environmental microbiology reports·2026
Same author

Identification of a sex-determining region potentially involved in resolving genetic conflicts over sex ratio.

Biology letters·2025
Same author

Phylogenetic relatedness rather than aquatic habitat fosters horizontal transfer of transposable elements in animals.

Genome research·2025
Same author

The Genetics of Host Plant Acceptance in Pea Aphids.

Molecular ecology·2025
Same author

Self-documenting plasmids.

Trends in biotechnology·2025

Related Experiment Video

Updated: Jul 2, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K

Cryptographic approaches to authenticating synthetic DNA sequences.

Casey-Tyler Berezin1, Samuel Peccoud2, Diptendu M Kar3

  • 1Department of Chemical & Biological Engineering, Colorado State University, Fort Collins, CO, USA.

Trends in Biotechnology
|February 28, 2024
PubMed
Summary

Synthetic DNA authenticity is crucial for the bioeconomy. DNA watermarking with digital signatures ensures sequence integrity, security, and traceability for synthetic DNA and GMOs.

Keywords:
DNA cryptographyDNA watermarkcyberbiosecuritydigital signaturegenetic engineering attribution

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

968
DNA Stable-Isotope Probing DNA-SIP
14:57

DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

44.2K

Related Experiment Videos

Last Updated: Jul 2, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

968
DNA Stable-Isotope Probing DNA-SIP
14:57

DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

44.2K

Area of Science:

  • Biotechnology and Synthetic Biology
  • Bioinformatics and Computational Biology
  • Molecular Biology

Background:

  • The growing bioeconomy relies heavily on synthetic DNA sequences.
  • Ensuring the authenticity and integrity of synthetic DNA is critical for its reliable application.
  • Current methods may lack robust security, error correction, and public verifiability.

Purpose of the Study:

  • To explore the application of DNA watermarking and digital signature techniques for synthetic DNA.
  • To address challenges in translating these techniques to complex genetically modified organisms (GMOs).
  • To advocate for the widespread implementation of these technologies to enhance security and traceability.

Main Methods:

  • Encoding identifying data into short DNA sequences (watermarking).
  • Integrating error correction and encryption protocols for robustness and security.
  • Utilizing novel digital signature techniques for public verification of sequence integrity.
  • Adapting techniques for application in genetically modified organisms (GMOs).

Main Results:

  • DNA watermarks can encode data, improving sequence security and robustness.
  • Digital signatures enable public verification against modification.
  • Synthetic DNA can be made self-documenting with sufficient information.
  • Considerations for applying these methods to GMOs are identified.

Conclusions:

  • DNA watermarking and digital signatures offer a robust solution for synthetic DNA authenticity.
  • These techniques enhance authorship, traceability, and detection of unauthorized use.
  • Widespread implementation is recommended for secure and verifiable synthetic DNA applications in the bioeconomy and GMOs.