Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

18.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Cesspools of Chichester: A Courageous Speech.

The Hospital·2018
Same author

Influence of protein supplements on the secretion of leukaemia inhibitory factor by mitomycin-pretreated Vero cells: possible application to the in vitro production of bovine blastocysts with high cryotolerance.

Journal of reproduction and fertility·2000
Same author

Evaluation of mitomycin-treated vero cells as a co-culture system for IVM/IVF-derived bovine embryos.

Theriogenology·1997
Same author

Evaluation of culture systems containing bovine oviduct epithelial cells or granulosa cells to mature and maintain the developmental competence of bovine oocytes in vitro.

Theriogenology·1994
Same author

Development of whole and demi-embryos of mice in culture and in vivo after supercooled storage.

Cryobiology·1993
Same author

Parthenogenetic development of activated in vitro matured bovine oocytes.

Theriogenology·1993
Same journal

Steroidal alkaloids as multi-target therapeutics for the treatment of endometriosis.

Steroids·2026
Same journal

Emerging trends for therapeutic valuation of Cyclopamine: Structural features, mechanism of action, and formulation strategies.

Steroids·2026
Same journal

The antiproliferative and antimigratory properties of the 3-biotin-B-norcholesteryl benzimidazole derivative in MCF-7 cells are mediated through G1 phase arrest and apoptosis.

Steroids·2026
Same journal

Development and validation of an LC-MS method for quantification of sex steroid hormones in skeletal muscle.

Steroids·2026
Same journal

Circulating 4β-hydroxycholesterol as a biomarker of CYP3A4 activity: beyond drug development.

Steroids·2026
Same journal

Impact of glycosylation position on flavonoid isomers' inhibition of breast cancer-associated enzyme CYP1B1.

Steroids·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

9.9K

A simple enzyme-linked immunosorbent assay for testosterone.

G J Marcus, R Durnford

    Steroids
    |December 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    A novel enzyme-linked immunosorbent assay for testosterone offers a convenient, rapid, and reliable alternative to traditional radioimmunoassays. This new method provides comparable sensitivity and accuracy for testosterone measurement.

    More Related Videos

    Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
    07:30

    Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

    Published on: May 10, 2018

    9.8K
    Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
    06:13

    Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

    Published on: November 24, 2010

    35.8K

    Related Experiment Videos

    Last Updated: Mar 14, 2026

    Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
    06:15

    Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

    Published on: September 7, 2018

    9.9K
    Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
    07:30

    Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

    Published on: May 10, 2018

    9.8K
    Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
    06:13

    Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

    Published on: November 24, 2010

    35.8K

    Area of Science:

    • Endocrinology
    • Biochemistry
    • Assay Development

    Background:

    • Radioimmunoassay (RIA) has been a standard for testosterone measurement but involves radioactive materials and complex procedures.
    • Existing non-radioactive testosterone assays may lack the sensitivity, reliability, or speed required for clinical or research applications.

    Purpose of the Study:

    • To develop and describe a new, non-radioactive enzyme-linked immunosorbent assay (ELISA) for quantifying testosterone.
    • To evaluate the performance characteristics of the novel testosterone ELISA, comparing it to established methods.

    Main Methods:

    • The assay utilizes horseradish peroxidase (HRP) conjugated to testosterone as the enzyme tracer.
    • Immune complex formation and subsequent enzymatic reaction are measured spectrophotometrically.

    Main Results:

    • The developed ELISA demonstrates comparable sensitivity and reliability to radioimmunoassay for testosterone detection.
    • The new assay is significantly simpler and faster to perform compared to previously reported testosterone assays.

    Conclusions:

    • This enzyme-linked immunosorbent assay represents a practical and efficient method for testosterone measurement.
    • The assay offers a convenient, sensitive, and reliable alternative to radioimmunoassay, suitable for various laboratory settings.