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Development and experimental validation of an M2 macrophage and platelet-associated gene signature to predict

Bahaerguli Muhuitijiang1, Jiawei Zhou1, Ranran Zhou1

  • 1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Cancer Science
|February 29, 2024
PubMed
Summary

Platelets promote M2 macrophage polarization in bladder cancer (BLCA). A new gene signature involving platelets and M2 macrophages aids in predicting prognosis and guiding personalized immunotherapy for BLCA patients.

Keywords:
M2 macrophagebladder cancerimmunotherapyplateletprognosis

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Area of Science:

  • Oncology
  • Immunology
  • Genetics

Background:

  • Platelets and M2 macrophages are implicated in tumorigenesis.
  • The specific relationship between platelets, M2 macrophages, and bladder cancer (BLCA) prognosis is not well understood.

Purpose of the Study:

  • To investigate the interaction between platelets and M2 macrophages in BLCA.
  • To identify genes associated with platelets and M2 macrophages for prognostic prediction in BLCA.
  • To develop a gene signature for evaluating prognosis and predicting immunotherapeutic sensitivity in BLCA.

Main Methods:

  • In vitro experiments to assess platelet-M2 macrophage interactions.
  • Bioinformatic analysis to identify relevant genes (A2M, TGFB3, MYLK).
  • Construction and validation of a gene signature for prognosis prediction.

Main Results:

  • Platelets stimulated by BLCA cell lines promote M2 macrophage polarization.
  • Platelet infiltration is significantly associated with M2 macrophage infiltration in BLCA samples.
  • A novel gene signature comprising A2M, TGFB3, and MYLK was developed and validated.

Conclusions:

  • Platelets and M2 macrophages play a significant role in BLCA progression.
  • The developed gene signature can predict patient prognosis and immunotherapeutic sensitivity.
  • This signature may guide personalized treatment strategies for bladder cancer.