Glutathione detection in water and milk using a new probe DCYP based on benzopyranonitrile

Glutathione (GSH) is a potent antioxidant, fragrance, and anti-browning agent in the field of food chemistry. The accurate GSH evaluation in food and vegetables is critical for instructing the right supplementation of GSH in body. However, most reported GSH fluorescent probes were utilized for the biological imaging. In this study, a new probe DCYP-GSH was developed by coupling of benzopyranonitrile as signal reporter to N-methylpyridine through C = C bond as binding site. Notably, a significant increase in fluorescence intensity and a λ_max red-shift of DCYP-GSH in electron spectra were found as a result of the response to GSH. Quantitative detection of GSH in water and milk samples were achieved using probe DCYP-GSH. The development of DCYP-GSH was anticipated to provide an effective toolkit for food safe evaluation.