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Updated: May 21, 2026

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
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Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

Published on: February 2, 2017

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Stepwise targeted strategies for improving neurological function by inhibiting oxidative stress levels and

Yi Li1, Jun Liao2, Liyan Xiong3

  • 1Shanghai Engineering Research Center of Organ Repair, School of Medicine, or Materials Science and Engineering, Shanghai University, Shanghai 200444, China; Department of Pharmacy, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, China.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|February 29, 2024
PubMed

Insights

New nanoparticles target the brain to treat ischemic stroke by reducing inflammation and protecting neurons. This drug delivery strategy shows promise for managing stroke and preventing further damage.

Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Nanotechnology

Background:

  • Ischemia-reperfusion injury causes neuronal damage due to reactive oxygen species (ROS) and inflammation, leading to blood-brain barrier (BBB) dysfunction.
  • Current treatments for ischemic stroke are limited, highlighting the need for novel therapeutic strategies.
  • Effective drug delivery across the BBB to target damaged brain regions remains a significant challenge.

Purpose of the Study:

  • To develop a multifunctional nanoparticle (ORD@SHp@ANG) for targeted drug delivery in ischemic stroke.
  • To evaluate the ability of these nanoparticles to cross the blood-brain barrier and target ischemic sites.
  • To assess the therapeutic efficacy of the nanoparticles in reducing oxidative stress, inflammation, and neuronal damage.

Main Methods:

  • Preparation of multifunctional nanoparticles (ORD@SHp@ANG) incorporating stroke-homing and BBB-targeting peptides, and ROS-responsive components.
  • In vitro assessment of ROS scavenging activity and protection of neuronal cells (SH-SY5Y) against oxidative damage.
  • In vivo evaluation in a rat ischemia-reperfusion model to assess BBB crossing, anti-inflammatory effects, reduction of cerebral infarction, and neuronal apoptosis.

Main Results:

  • ORD@SHp@ANG nanoparticles successfully crossed the BBB and selectively targeted ischemic brain areas.
  • Nanoparticles demonstrated effective ROS scavenging in vitro and protected neuronal cells.
  • In vivo studies showed reduced cerebral infarction, suppressed inflammation, preserved BBB integrity, and decreased neuronal apoptosis in a rat stroke model.

Conclusions:

  • ORD@SHp@ANG nanoparticles represent a promising drug delivery system for ischemic stroke management.
  • The strategy effectively targets the ischemic brain, scavenges ROS, and modulates neuroinflammation.
  • This approach offers a potential therapeutic solution for reducing neuronal damage and improving outcomes after ischemic stroke.
Keywords:
Blood–brain barrier integrityDanshensuIschemia-reperfusion injuryNeuroprotectionStepwise targeted drug delivery strategy

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