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Published on: March 10, 2015
Pre and Post-high-intensity Interval Training Delays Colon Tumor Onset in a Syngeneic Mouse Model
Eduardo Figueiredo Silva1, Bruno Nappo Fernandes1, Poliana Marinello2
1Molecular Carcinogenesis Program, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil.
Background/Aim:
High-intensity interval training (HIIT) can trigger transient anti-tumor cytotoxicity through the mobilization of natural killer cells (NK cells) and myokines. Yet, the effects of HIIT on tumor development and microenvironment are unclear.
Materials And Methods:
Male C57/BL6 mice were administered either MC38 of syngeneic colon cancer cells or vehicle in a single subcutaneous injection. Before injection, the training group completed four weeks of the HIIT program (progressive swimming training, 3/week, 10-12 min, 4-6% of body weight for overload). Following injection, trained mice continued to exercise for two additional weeks.
Results:
Pre and post-HIIT training was effective in preventing tumor onset (p=0.0065), maintaining body weight gain, and counteracting splenomegaly by 40% compared to the tumor group. However, HIIT had no impact on suppressing tumor growth, modifying final tumor volume, or significantly changing tumor proliferation (Ki-67), connective tissue content, or DNA double-strand damage detected by phospho-histone gamma-H2AX (γ-H2AX).
Conclusion:
Pre and post-HIIT program is feasible for mice carrying a subcutaneous syngeneic tumor and effective in delaying tumor burden; however, HIIT did not alter colon tumor endpoints.
