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Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

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Temporal changes in ewe vaginal microbiota throughout gestation.

Mackenzie S Cassas¹, Lucille C Jonas^1,2, Chiron J Anderson^1,2

¹Department of Animal Science, Iowa State University, Ames, IA, United States.

Frontiers in Microbiology

|March 1, 2024

Summary

This summary is machine-generated.

The ewe vaginal microbiome changes significantly during pregnancy, with distinct bacterial shifts observed across early, transitional, and late gestation periods. These changes may be linked to hormonal fluctuations and could offer insights into sheep reproductive success.

Keywords:

16S rRNA gene amplicon sequencing Illumina MiSeq pregnancy sheep vaginal microbiota

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Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

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Published on: October 25, 2015

Area of Science:

Veterinary Science
Microbiology
Reproductive Biology

Background:

Sheep reproductive efficiency is influenced by numerous factors.
The role of the vaginal microbiota in sheep reproductive success remains understudied.
Characterizing the ewe vaginal microbiota throughout pregnancy is crucial for understanding reproductive outcomes.

Purpose of the Study:

To thoroughly characterize the ewe vaginal microbiota throughout the course of pregnancy.
To identify shifts in bacterial communities during different gestational stages.
To explore potential links between vaginal microbiota and hormonal changes during gestation.

Main Methods:

Vaginal samples were collected weekly from 31 pregnant ewes from pre-breeding to post-lambing.
DNA was extracted from samples for 16S rRNA gene Illumina MiSeq amplicon sequencing.
Alpha diversity metrics and relative abundance of bacterial taxa were analyzed.

Main Results:

Vaginal microbial diversity increased throughout gestation.
Three distinct periods of bacterial community shifts were identified: early, transitional, and mid/late gestation.
Specific bacterial genera like *Actinobacillus*, *Histophilus*, *Streptococcus*, *Staphylococcus*, and *Ureaplasma* showed varying abundances across these periods.

Conclusions:

Vaginal microbiota composition in ewes undergoes significant dynamic changes during pregnancy.
These shifts are potentially influenced by hormonal changes, particularly progesterone levels.
Understanding these microbial dynamics can aid in identifying biomarkers for pregnancy outcomes and developing probiotics to enhance sheep reproductive success.