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Updated: Jul 18, 2026

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Carbapenem-resistant Acinetobacter baumannii carrier detection: a simple and efficient protocol.

Jonathan Lellouche^1,2, Alona Keren-Paz¹, Reut Rov¹

¹National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, Tel Aviv, Israel.

Microbiology Spectrum

|March 1, 2024

Summary

A new protocol using skin sampling and modified CHROMagar Acinetobacter (mCaA) effectively detects carbapenem-resistant Acinetobacter baumannii (CRAB) carriers. This method offers a rapid and reliable 24-hour turnaround for infection control.

Keywords:

carbapenem-resistant Acinetobacter baumannii carrier detection screening

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Area of Science:

Clinical Microbiology
Infectious Diseases
Molecular Diagnostics

Background:

Carbapenem-resistant Acinetobacter baumannii (CRAB) is a critical multidrug-resistant pathogen causing significant nosocomial infections.
Standardized laboratory methods for CRAB carrier identification are lacking, hindering effective infection control strategies.
WHO and CDC highlight CRAB as a major public health threat requiring urgent detection and management.

Purpose of the Study:

To develop and validate a practical, sensitive, and specific protocol for detecting CRAB carriers from screening samples.
To compare the performance of different selective agar media for CRAB isolation.
To optimize sample enrichment time and identify the most accurate PCR-based methods for CRAB identification.

Main Methods:

Evaluated three selective agar media: mSuperCARBA (SC), CHROMagar Acinetobacter (CaA), and modified CHROMagar Acinetobacter (mCaA) with meropenem.
Assessed PCR for species-specific genes (gyrB, blaOXA-51) and carbapenemase genes (blaOXA-23, blaOXA-24, blaOXA-58).
Conducted a prospective study using skin sampling with a 6-hour enrichment period, followed by plating on mCaA and PCR confirmation.

Main Results:

Modified CHROMagar Acinetobacter (mCaA) demonstrated high specificity (97%) and sensitivity (98%) for CRAB detection.
Skin sampling with a 6-hour enrichment achieved 98% sensitivity for CRAB carriage detection.
PCR targeting gyrB (98% accuracy) and blaOXA-51 (99% accuracy) effectively differentiated CRAB from other species on mCaA.

Conclusions:

A novel protocol combining skin sampling, short enrichment, mCaA selection, and PCR-based identification provides reliable and rapid CRAB carrier detection.
This protocol achieves a 24-hour turnaround time, facilitating timely implementation of infection control measures.
The developed method addresses the need for standardized and efficient CRAB screening in healthcare settings.