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SIRT2-mediated deacetylation of ACLY promotes the progression of oesophageal squamous cell carcinoma.

Xueying Zhang1, Yue Xu2, Shenglei Li2

  • 1School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.

Journal of Cellular and Molecular Medicine
|March 1, 2024
PubMed
Summary

Targeting ATP citrate lyase (ACLY) inhibits esophageal squamous cell carcinoma (ESCC) progression by impacting lipid synthesis. The SIRT2/ACLY pathway presents a potential therapeutic strategy for ESCC treatment.

Keywords:
ATP citrate lyaseBMS-303141acetylationlipid metabolismoesophageal squamous cell carcinomasirtuin 2

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Area of Science:

  • Oncology
  • Biochemistry
  • Molecular Biology

Background:

  • ATP citrate lyase (ACLY) is crucial for lipid metabolism and biosynthesis in various cancers.
  • ACLY's role in esophageal squamous cell carcinoma (ESCC) is not well understood.
  • ACLY is frequently overexpressed in tumors, and its inhibition can impede cancer progression.

Purpose of the Study:

  • To investigate the function of ACLY in esophageal squamous cell carcinoma (ESCC).
  • To explore the potential of targeting the SIRT2/ACLY signaling axis for ESCC therapy.

Main Methods:

  • Utilized ACLY inhibitors and overexpression in ESCC cell lines.
  • Assessed cell proliferation, migration, invasion, and lipid synthesis.
  • Employed xenograft tumor models in mice.
  • Investigated protein acetylation and interaction with SIRT2, using SIRT2 inhibitor AGK2.

Main Results:

  • ACLY inhibition reduced ESCC cell proliferation, migration, invasion, and lipid synthesis.
  • ACLY overexpression enhanced these processes in ESCC.
  • ACLY inhibition suppressed tumor growth in vivo.
  • ACLY interacts with SIRT2; SIRT2 inhibition increased ACLY acetylation and inhibited ESCC cell growth, an effect partially reversed by ACLY overexpression.

Conclusions:

  • ACLY plays a significant role in ESCC proliferation, migration, invasion, and lipid metabolism.
  • The SIRT2/ACLY signaling axis is a potential therapeutic target for esophageal squamous cell carcinoma.
  • Targeting this axis may offer a novel treatment strategy for ESCC patients.