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Nutrient consumption patterns of Lactobacillus acidophilus.
Yali Qiao1, Boxing Yin2, Wei Zhou2
1Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, China.
Journal of the Science of Food and Agriculture
|March 1, 2024
Summary
Certain nutrients like alanine and guanine significantly enhance the survival of Lactobacillus acidophilus (L. acidophilus) under acidic conditions. This research identifies key substances to improve probiotic efficacy in acidic environments.
Area of Science:
- Microbiology
- Probiotics
- Nutritional Science
Background:
- Probiotic efficacy of Lactobacillus acidophilus (L. acidophilus) is limited by acid stress.
- Identifying factors that enhance L. acidophilus acid resistance is crucial for its application.
Purpose of the Study:
- To identify specific nutrients that improve the acid resistance of L. acidophilus.
- To understand nutrient consumption patterns of L. acidophilus under varying pH levels.
Main Methods:
- Assessed nutrient consumption patterns of L. acidophilus LA-5 at different pH levels (5.8, 4.9, 4.4).
- Quantified the impact of individual nutrient supplementation on L. acidophilus viable counts under acid stress.
Main Results:
- Alanine, uracil, adenine, guanine, niacin, and manganese showed high consumption rates (>60%) at pH 4.4-5.8.
- Glutamic acid + glutamine and thiamine consumption increased as pH decreased.
- Supplementation with alanine, glutamic acid + glutamine, guanine, niacin, thiamine, or manganese significantly increased L. acidophilus viable counts under acid stress.
Conclusions:
- Alanine, glutamic acid + glutamine, guanine, niacin, thiamine, and manganese enhance L. acidophilus growth in acidic conditions.
- Findings provide strategies for optimizing L. acidophilus acid resistance for industrial applications.
