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Silencing circLDLRAD3 Inhibits Lung Cancer Progression by Regulating the miR-497-5p/PFKP Axis

Hong Zhou1, Rui Wu1, Hong Li2

  • 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yantaxi Road, Xi'an, 710061, Shaanxi, China.

Molecular Biotechnology
|March 1, 2024
PubMed
Abstract

Insights

Circular RNA circLDLRAD3 promotes lung cancer by increasing PFKP via sponging miR-497-5p. Silencing circLDLRAD3 inhibits cancer progression and tumor growth, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lung cancer is a leading global cause of mortality.
  • Circular RNAs (circRNAs) are increasingly recognized as crucial regulators in various cancers.
  • The specific role and mechanism of circRNAs, like circLDLRAD3, in lung cancer remain largely undefined.

Purpose of the Study:

  • To elucidate the functional mechanism of circLDLRAD3 in lung cancer.
  • To investigate the regulatory relationship between circLDLRAD3, microRNA-497-5p (miR-497-5p), and platelet-type PFK (PFKP) in lung cancer progression.

Main Methods:

  • Quantification of circLDLRAD3, miR-497-5p, and PFKP expression using RT-qPCR and Western blot.
  • In vitro functional assays including CCK-8, EdU, Transwell, wound healing, and flow cytometry.
  • Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays to determine molecular interactions; in vivo tumor growth studies.

Main Results:

  • circLDLRAD3 and PFKP levels were elevated in lung cancer tissues.
  • circLDLRAD3 knockdown significantly suppressed cell viability, proliferation, migration, invasion, and glycolysis, while promoting apoptosis.
  • circLDLRAD3 acts as a sponge for miR-497-5p, leading to increased PFKP expression; miR-497-5p inhibits lung cancer progression by targeting PFKP. circLDLRAD3 knockdown inhibited tumor growth in vivo.

Conclusions:

  • circLDLRAD3 promotes lung cancer development by upregulating PFKP expression through sponging miR-497-5p.
  • The circLDLRAD3/miR-497-5p/PFKP axis represents a novel regulatory pathway in lung cancer.
  • circLDLRAD3 inhibition presents a potential targeted therapeutic strategy for lung cancer treatment.
Keywords:
Lung cancerPFKPcircLDLRAD3miR-497-5p

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