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Platelet dysfunction reversal with cold-stored vs room temperature-stored platelet transfusions
Valery J Kogler1,2, Jeffrey A Miles1,3, Tahsin Özpolat1,4
1Bloodworks Northwest Research Institute, Seattle, WA.
Blood
|March 1, 2024
Summary
Room temperature-stored platelets (RSPs) better reversed platelet inhibition, while cold-stored platelets (CSPs) increased thrombin generation. Donor factors predict efficacy for both RSPs and extended-stored CSPs.
Area of Science:
- Transfusion Medicine
- Hematology
- Immunology
Background:
- Platelets stored at room temperature (RSPs) have a 5-7 day shelf life.
- To address shortages, 14-day cold-stored platelets (CSPs) are now approved.
- The post-transfusion function and ideal donor profiles for CSPs remain unclear.
Purpose of the Study:
- To evaluate post-transfusion platelet function of 7-day RSPs and 14-day CSPs.
- To identify predictors of platelet function for both storage methods.
- To compare RSPs and CSPs in healthy volunteers taking aspirin.
Main Methods:
- A randomized crossover study involving 10 healthy volunteers.
- Participants received both RSPs and CSPs sequentially after aspirin ingestion.
- Post-transfusion platelet function, thrombin generation, and lipid mediators were assessed.
Main Results:
- RSPs significantly improved platelet inhibition in integrin activation assays.
- CSPs led to significantly higher thrombin generation, independent of microparticles.
- Lysophosphatidylcholine-O species predicted CSP procoagulant capacity; fatty acids predicted RSP aggregation.
Conclusions:
- This study provides initial efficacy data for extended-stored CSPs.
- Identifying suitable donors for both RSPs and CSPs appears feasible.
- Findings support further investigation in larger clinical trials.
