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Updated: Jul 1, 2025
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
Meta-analysis to determine efficacy of colony-stimulating factor 2 for improving pregnancy success after embryo
Peter J Hansen1, Eliab Estrada-Cortés2, Thiago F Amaral1
1Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, 32611-0910, FL, USA.
Abstract:
Results have been inconsistent as to whether addition of colony stimulating factor 2 (CSF2) to culture medium improves embryo competence for establishment of pregnancy in cattle and humans. The purpose of the current study was to use all available experiments in cattle concerning effects of CSF2 on pregnancy success after transfer into recipient cattle. The approach was to perform a meta-analysis of all published data sets as well as data from an unpublished experiment described for the first time here. Meta-analysis failed to support the hypothesis that addition of CSF2 to embryo culture medium improves competence of bovine blastocysts to increase pregnancy or calving rates after transfer into recipient females. Thus, its general use as a culture medium additive to increase pregnancy success after embryo transfer is not recommended.
