Related Experiment Video
Updated: Jul 1, 2025

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
Published on: July 16, 2021
Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
Satoshi Yamazaki1, Yo Mabuchi2, Takaharu Kimura1
1Laboratory of Stem Cell Therapy, Faculty of Medicine, University of Tsukuba, Ibaraki 305-8575, Japan.
Abstract:
Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα+Sca-1+ cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation.
Related Concept Videos
Mesenchymal Stem Cells
Differentiation of Common Myeloid Progenitor Cells
Regulation of Hematopoietic Stem Cells
Stem Cell Niche
Multipotency of Hematopoietic Stem Cells

