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Venipuncture Site Influences Blood-drop Volume in C57BL/6 Mice
Elizabeth S Lavin1, Erica R Feldman1, Scott M Soprano1
1Center for Animal Resources and Education, Cornell University, Ithaca, New York.
Summary
This study experimentally determined mouse blood drop volumes, finding facial vein draws yield the largest drops (21.7μL) but increase risk. Saphenous and tail veins offer safer, smaller volumes for serial sampling.
Area of Science:
- Animal research methodology
- Laboratory animal science
- Biomedical research techniques
Background:
- Serial blood collection in mice is crucial for experiments.
- Current methods often estimate blood volume by drops, using an anecdotal 25μL conversion factor.
- Accurate volume estimation is vital for IACUC compliance and animal welfare.
Purpose of the Study:
- To experimentally determine the actual volume of a single drop of mouse blood from different venipuncture sites.
- To compare blood drop volumes obtained from facial, saphenous, and tail veins.
- To provide data for more accurate estimations of blood loss in mouse studies.
Main Methods:
- A 10-week crossover study involving 30 C57BL/6J mice.
- Blood drops were collected from facial, saphenous, and tail veins using different needle gauges.
- Drop weight was measured, and volume calculated using determined blood density.
Main Results:
- Venipuncture site significantly impacted blood drop volume.
- Facial vein puncture yielded the largest drops (mean 21.7μL), followed by saphenous (9.97μL) and tail veins (4.96μL).
- Facial vein collection was linked to increased hemorrhage and morbidity.
Conclusions:
- The actual volume of a mouse blood drop varies significantly by collection site.
- Saphenous and tail veins minimize blood loss, optimizing serial sampling and animal welfare.
- This data allows for more precise blood volume calculations, improving experimental accuracy and compliance.
