The novel circRNA circ_0045881 inhibits cell proliferation and invasion by targeting mir-214-3p in triple-negative breast cancer
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View abstract on PubMed
Summary
This summary is machine-generated.Triple-negative breast cancer (TNBC) progression is linked to decreased hsa_circ_0045881. This circular RNA acts as a sponge for miR-214-3p, inhibiting TNBC cell invasion, migration, and proliferation, suggesting it as a potential therapeutic target.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype.
- The circRNA-miRNA-mRNA axis is crucial for breast cancer diagnosis and prognosis.
- Key circRNAs and regulatory mechanisms in TNBC progression are not well understood.
Purpose Of The Study
- To identify critical circRNAs involved in TNBC progression.
- To elucidate the regulatory mechanisms of these circRNAs in TNBC.
- To evaluate the potential of identified circRNAs as biomarkers or therapeutic targets for TNBC.
Main Methods
- CircRNA microarray analysis of TNBC patient tissues.
- Bioinformatic analyses (GO, KEGG) to identify significant circRNAs.
- In vitro assays (Transwell, wound healing, CCK8) to assess cell invasion, migration, and proliferation.
- Molecular assays (dual luciferase, RIP) to confirm circRNA-miRNA interactions.
Main Results
- Identified 78 differentially expressed circRNAs in TNBC tissues.
- Found significantly decreased hsa_circ_0045881 levels in TNBC.
- Demonstrated that hsa_circ_0045881 overexpression inhibits TNBC cell invasion and migration.
- Confirmed hsa_circ_0045881 acts as a sponge for miR-214-3p, which promotes TNBC cell invasion, migration, and proliferation.
Conclusions
- hsa_circ_0045881 plays a critical role in TNBC progression.
- hsa_circ_0045881 regulates TNBC cell invasion, metastasis, and proliferation by sponging miR-214-3p.
- hsa_circ_0045881 is a potential prognostic marker and therapeutic target for TNBC.
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