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The circRNA hsa-circ-0013561 regulates head and neck squamous cell carcinoma development via the miR-7-5p/PDK3 axis

Kaisai Tian1,2, Liying Zheng1,2, Tailei Yuan1

  • 1Postgraduate Training Base at Shanghai Gongli Hospital, Ningxia Medical University, Shanghai, 200135, China.

Cancer Cell International
|March 1, 2024
PubMed
Abstract

Insights

Downregulating hsa-circ-0013561 inhibits head and neck squamous cell carcinoma (HNSCC) progression and metastasis. This occurs by modulating miR-7-5p binding and PDK3 expression, impacting cell proliferation and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in cancer progression.
  • Hsa-circ-0013561 is abnormally expressed in head and neck squamous cell carcinoma (HNSCC).
  • The precise role of hsa-circ-0013561 in HNSCC progression remains unclear.

Purpose of the Study:

  • To investigate the role of hsa-circ-0013561 in HNSCC progression and metastasis.
  • To identify the downstream targets and regulatory mechanisms of hsa-circ-0013561 in HNSCC.

Main Methods:

  • Fluorescence in situ hybridization (FISH) and qRT-PCR were used to assess hsa-circ-0013561 expression.
  • Dual-luciferase reporter assays identified downstream targets.
  • Transwell migration, EDU, CCK8, and colony formation assays evaluated cell behavior.
  • A mouse xenograft model assessed in vivo effects on tumor progression and metastasis.

Main Results:

  • Hsa-circ-0013561 was found to be upregulated in HNSCC tissues.
  • Downregulation of hsa-circ-0013561 inhibited HNSCC cell proliferation and migration, promoting apoptosis and G1 cell cycle arrest.
  • miR-7-5p and PDK3 were identified as downstream targets.
  • PDK3 overexpression or miR-7-5p suppression reversed the inhibitory effects of hsa-circ-0013561 downregulation.

Conclusions:

  • Hsa-circ-0013561 downregulation inhibits HNSCC metastasis and progression.
  • The mechanism involves modulating PDK3 expression and miR-7-5p binding.
  • This study elucidates a novel regulatory pathway for HNSCC.
Keywords:
Head and neck squamous cell carcinoma (HNSCC)Hsa-circ-0013561PDK3miR-7-5p

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