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Related Concept Videos

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  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
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  11. Near-infrared-ii Fluorescence/magnetic Resonance Double Modal Imaging Of Transplanted Stem Cells Using Lanthanide Co-doped Gadolinium Oxide Nanoparticles.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Near-infrared-ii Fluorescence/magnetic Resonance Double Modal Imaging Of Transplanted Stem Cells Using Lanthanide Co-doped Gadolinium Oxide Nanoparticles.

Related Experiment Video

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
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Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice

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Near-infrared-II fluorescence/magnetic resonance double modal imaging of transplanted stem cells using lanthanide co-doped gadolinium oxide nanoparticles.

Shota Yamada1, Kaori Yamada2, Ayae Sugawara-Narutaki3

  • 1Department of Energy Engineering, Graduate School of Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, 464-8603, Japan. yamada.shota.b6@s.mail.nagoya-u.ac.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|March 2, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
Adipose tissue-derived stem cell (ASCs)In vivo imagingLanthanide co-doped gadolinium oxide nanoparticleMagnetic nanoparticles

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Related Experiment Videos

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
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Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice

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New nanoparticles enable sensitive in vivo tracking of transplanted stem cells. This dual near-infrared-II fluorescence and MRI imaging technique improves monitoring of stem cell behavior for enhanced therapeutic safety and efficacy.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Regenerative Medicine

Background:

  • Accurate monitoring of transplanted stem cells is crucial for therapeutic success.
  • Conventional in vivo imaging lacks the sensitivity to detect stem cells effectively.
  • Understanding stem cell kinetics (behavior, accumulation, engraftment) is vital for safety and efficacy.

Purpose of the Study:

  • To develop a sensitive in vivo imaging technique for transplanted stem cells.
  • To utilize multifunctional nanoparticles for dual-modal imaging.
  • To assess the safety and efficacy of novel nanoparticles for stem cell labeling.

Main Methods:

  • Preparation of gadolinium oxide (Gd2O3) nanoparticles co-doped with Erbium (Er3+) and Ytterbium (Yb3+).
  • Transduction of nanoparticles into adipose tissue-derived stem cells (ASCs) via incubation, assessing cytotoxicity and morphological impact.
  • In vivo subcutaneous transplantation of labeled ASCs in mice.
  • Dual-modal imaging using near-infrared-II (NIR-II) fluorescence imaging and magnetic resonance imaging (MRI).

    • Main Results:

    • Gd2O3:Er,Yb nanoparticles were successfully prepared and transduced into ASCs without cytotoxicity or morphological changes.
    • Labeled ASCs were effectively imaged in vivo using a combined NIR-II fluorescence and MRI system.
    • The dual-modal imaging approach provided good contrast for visualizing transplanted stem cells.

    Conclusions:

    • Gd2O3:Er,Yb nanoparticles are suitable for labeling stem cells for in vivo imaging.
    • The developed dual-modal imaging technique offers high sensitivity for tracking transplanted stem cells.
    • This approach holds potential for improving the safety and efficacy of stem cell transplantation therapies.