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Acquired Pellicle and Biofilm Engineering by Rinsing with Hemoglobin Solution
Tamara T Araujo1, Thamyris S Carvalho1, Aline Dionizio1
1Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Caries Research
|March 3, 2024
Summary
Hemoglobin (Hb) engineering of the acquired enamel pellicle (AEP) shows promise for preventing dental caries. This study found Hb modified the oral microbiome and reduced enamel demineralization, suggesting its potential in anticaries products.
Area of Science:
- Biomaterials Science
- Microbiology
- Dental Research
Background:
- The acquired enamel pellicle (AEP) can be engineered by incorporating proteins like hemoglobin (Hb) to prevent dental demineralization.
- This
Purpose of the Study:
- To investigate the effects of Hb-engineered AEP on the oral biofilm microbiome and enamel demineralization using in vivo and in vitro models.
Main Methods:
- In vivo: Volunteers rinsed with Hb solution; AEP and biofilm were collected for proteomics and 16S-rRNA sequencing.
- In vitro: Bovine enamel specimens were treated with varying Hb concentrations and exposed to biofilm; analyses included resazurin, CFU, and microradiography.
Main Results:
- Hb incorporation into AEP increased beneficial proteins (antioxidant, antimicrobial, acid-resistance) and reduced pathogenic bacteria.
- In vitro, the lowest Hb concentration effectively reduced bacterial activity, CFU, and enamel demineralization compared to controls.
Conclusions:
- Hemoglobin (Hb) shows potential for use in anticaries dental products.
- Hb can modify the oral microbiome and control caries by engineering the AEP and biofilm.
