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Related Experiment Video

Updated: Jul 1, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
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The tumor suppressor p53 is a negative regulator of the carcinoma-associated transcription factor FOXQ1.

Giulia Pizzolato1, Lavanya Moparthi1, Pierfrancesco Pagella1

  • 1Wallenberg Centre for Molecular Medicine (WCMM), Linköping University, Linköping, Sweden; Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.

The Journal of Biological Chemistry
|March 3, 2024
PubMed
Summary

The tumor suppressor p53 negatively regulates FOXQ1 expression, a factor driving cancer metastasis. Loss of p53 function in cancer leads to increased FOXQ1, promoting tumor progression.

Keywords:
FOXQ1GloProcolorectal cancerforkhead boxgene regulationgene transcriptionp53proteomics

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Area of Science:

  • Molecular biology
  • Cancer research
  • Genetics

Background:

  • Forkhead box Q1 (FOXQ1) is upregulated in carcinomas, promoting epithelial-to-mesenchymal transition and metastasis.
  • Mechanisms of FOXQ1 deregulation in cancer are not fully understood.

Purpose of the Study:

  • To identify transcriptional regulators of FOXQ1.
  • To elucidate the role of p53 in FOXQ1 expression and its implications in cancer.

Main Methods:

  • CRISPR-Cas9-based genomic locus proteomics.
  • Promoter reporter assays.
  • Chromatin immunoprecipitation followed by quantitative PCR (ChIP-qPCR).
  • Gain and loss-of-function assays.
  • Pharmacological activation of p53.

Main Results:

  • The tumor suppressor p53 was identified as a negative regulator of FOXQ1 expression.
  • p53 binds near the FOXQ1 promoter and suppresses its transcription.
  • Pharmacological p53 activation reduced FOXQ1 levels in wildtype p53 cancer cells.
  • p53 mutations correlate with increased FOXQ1 expression in human cancers.

Conclusions:

  • Loss of p53 function derepresses FOXQ1, contributing to tumor progression.
  • p53 acts as a critical suppressor of FOXQ1, impacting cancer metastasis.