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Skin Cancer

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...

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Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

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Lymphatic expression of the proliferation marker Ki67 is linked to sentinel node positivity, recurrence and mortality

Samuel X Tan¹, Sharene Chong¹, Casey Rowe¹

¹Frazer Institute, University of Queensland, Brisbane, Queensland, Australia.

Experimental Dermatology

|March 4, 2024

Summary

This summary is machine-generated.

Emergent lymphangiogenesis, indicated by D2-40+/Ki67+ co-expression, signals increased melanoma risk. This finding highlights lymphatic proliferation as a key indicator for melanoma-specific mortality and recurrence in primary cutaneous melanoma patients.

Keywords:

biomarker immunofluorescence melanoma pathology prognosis

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Published on: September 8, 2021

Area of Science:

Oncology
Dermatology
Pathology

Background:

Lymphangiogenesis, the formation of new lymphatic vessels, precedes lymphovascular invasion.
Lymphovascular invasion is linked to higher metastasis and mortality risks in primary cutaneous melanoma.
Identifying early indicators of melanoma progression is crucial for patient prognosis.

Purpose of the Study:

To investigate the association between emergent lymphangiogenesis and prognosis in primary cutaneous melanoma.
To evaluate if co-expression of endothelial proteins with Ki67 predicts poorer outcomes.
To determine the prognostic value of lymphatic proliferation in melanoma patients.

Main Methods:

Retrospective longitudinal study of 264 primary cutaneous melanoma patients.
Opal multiplex immunofluorescence staining for endothelial proteins (CD31, D2-40) and Ki67.
Multivariate Cox regression analysis to assess the association between endothelial Ki67 positivity and clinical outcomes.

Main Results:

Co-expression of D2-40+/Ki67+ was significantly associated with increased melanoma-specific mortality (aHR: 2.03) and recurrence (aHR: 1.70).
CD31+/Ki67+ co-expression did not show prognostic significance in this cohort.
Lymphatic proliferation, identified by D2-40+/Ki67+ co-expression, predicted poorer outcomes.

Conclusions:

Emergent lymphangiogenesis, specifically D2-40+/Ki67+ co-expression, is a significant predictor of adverse outcomes in primary cutaneous melanoma.
This marker of lymphatic proliferation may indicate a higher risk of metastasis and mortality.
D2-40+/Ki67+ co-expression can aid in risk stratification for melanoma patients.