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The Association of miRNA-146a Gene Variation and Multiple Sclerosis in The Iranian Population

Atefeh Zamani1, Masoumeh Dehghan Manshadi2, Mansoureh Akouchekian3

  • 1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Medical Journal of the Islamic Republic of Iran
|March 4, 2024
PubMed
Abstract

Insights

The miR-146a gene rs2910164 polymorphism is linked to multiple sclerosis (MS) susceptibility in Iranians. The rs2431697 polymorphism showed no association with MS in this population.

Area of Science:

  • Genetics
  • Immunology
  • Neurology

Background:

  • Multiple sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system.
  • MicroRNA-146a (miR-146a) is a non-coding RNA that regulates immune responses.

Purpose of the Study:

  • To investigate the association between miR-146a gene polymorphisms (rs2910164 and rs2431697) and MS risk in the Iranian population.

Main Methods:

  • Genotyping of rs2910164 and rs2431697 polymorphisms using PCR-RFLP and Tetra-primer ARMS-PCR.
  • Case-control study with 60 MS patients and 100 healthy controls.
  • Statistical analysis using the chi-squared test and Hardy-Weinberg equilibrium evaluation.

Main Results:

  • The miR-146a gene rs2431697 polymorphism was not associated with MS.
  • A significant association was found between the miR-146a gene rs2910164 polymorphism and MS (P = 0.012).

Conclusions:

  • The miR-146a gene rs2910164 polymorphism may contribute to MS susceptibility in the Iranian population.
  • Further research is warranted to elucidate the role of miR-146a in MS pathogenesis.
Keywords:
Multiple sclerosisPCR-RFLPTetra-primer ARMS-PCR.miR-146a

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