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Updated: Jul 14, 2026

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
Published on: January 30, 2013
A Chemically Inducible Muscle Ablation System for the Zebrafish
Eric Paulissen1, Benjamin L Martin1
1Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA.
Abstract:
An effective method for tissue-specific ablation in zebrafish is the nitroreductase (NTR)/metronidazole (MTZ) system. Expressing bacterial NTR in the presence of nitroimidazole compounds causes apoptotic cell death, which can be useful for understanding many biological processes. However, this requires tissue-specific expression of the NTR enzyme, and many tissues have yet to be targeted with transgenic lines that express NTR. We generated a transgenic zebrafish line expressing NTR in differentiated skeletal muscle. Treatment of embryos with MTZ caused muscle specific cell ablation. We demonstrate this line can be used to monitor muscle regeneration in whole embryos and in transplanted transgenic cells.
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