Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.3K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.3K
IR Spectrometers01:25

IR Spectrometers

1.1K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.1K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.0K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.0K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

391
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
391
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

219
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
219
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

645
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
645

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autonomously Motile Nano-PROTACs Act as Protein-Sweeping Robots to Enhance Targeted Protein Degradation.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

NAT10-mediated RNA N4-acetylation promotes intestinal fibroblast senescence via DHRS2.

Biochemical and biophysical research communications·2026
Same author

Spatiotemporal differentiation of Plasmodium vivax populations in the western Greater Mekong Subregion using a 22-SNP barcode.

PLoS neglected tropical diseases·2026
Same author

Does coil deployment improve outcomes in GOV1 gastroesophageal varices? A retrospective case-control study.

Surgical endoscopy·2026
Same author

<i>Plasmodium falciparum</i> genetic diversity in border areas of Myanmar assessed by genotyping merozoite surface protein genes.

Parasite epidemiology and control·2026
Same author

Chronic two-photon microscopy reveals neuronal activity patterns in the cerebral cortex of an Alzheimer's disease mouse model.

Biomedical optics express·2026

Related Experiment Video

Updated: Jul 1, 2025

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
10:13

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks

Published on: April 28, 2023

2.4K

Design method for engineering the initial structure of a spectrometer.

Zhaoqing Yang, Meng Xue, Hanming Guo

    Applied Optics
    |March 4, 2024
    PubMed
    Summary
    This summary is machine-generated.

    A new engineering initial structure method (MEIS) improves spectrometer design by considering component size and position, avoiding interference. This method offers a more rationalized and efficient approach compared to previous optical methods.

    More Related Videos

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
    13:31

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

    Published on: December 22, 2015

    15.1K
    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    10.2K

    Related Experiment Videos

    Last Updated: Jul 1, 2025

    A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
    10:13

    A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks

    Published on: April 28, 2023

    2.4K
    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
    13:31

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

    Published on: December 22, 2015

    15.1K
    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    10.2K

    Area of Science:

    • Optical engineering
    • Spectrometer design

    Background:

    • Traditional optical initial structure methods (MOIS) focus solely on optical properties, neglecting component dimensions.
    • This oversight leads to significant discrepancies between initial and optimized parameters, reducing the initial structure's practical value.

    Purpose of the Study:

    • To introduce a more efficient design method for engineering initial structure (MEIS) in spectrometers.
    • To incorporate physical constraints like component size and relative positioning to prevent optical element interference.

    Main Methods:

    • Deduction of anti-interference conditions using ray tracing.
    • Derivation of imaging formulas via geometric optics.
    • Rapid calculation of component parameters and initial structure acquisition with spacing margins.

    Main Results:

    • Successful design of a wide-band, high-resolution spectrometer (700-1000 nm, 0.5 nm resolution) using MEIS.
    • MEIS resulted in more rationalized component placement and eliminated complex optimization.
    • Minimal changes in image plane position, wheelbase (<0.5 mm), and deflection angle (0.5°) were observed.

    Conclusions:

    • MEIS provides a valuable reference for rapid and efficient spectrometer design.
    • The method ensures initial structures are physically feasible and avoid component interference.
    • MEIS significantly improves upon the limitations of previous optical-only design methods.