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Circ1811 suppresses gastric cancer progression by regulating the miR-632/DAPK1 axis

Min Fu1, Jianmei Gu2, Dan Yu3

  • 1Institute of Digestive Diseases, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, China; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

Gene
|March 4, 2024
PubMed

Insights

Circular RNAs (circRNAs) are key in cancer. This study found circ1811 suppresses gastric cancer (GC) by regulating the miR-632/DAPK1 pathway, suggesting its potential as a GC biomarker and therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized as critical regulators in cancer development.
  • The specific roles and mechanisms of circRNAs in gastric cancer (GC) are not fully understood.
  • This study focuses on circ1811, a novel circRNA with potential implications in GC.

Purpose of the Study:

  • To investigate the expression and function of circ1811 in gastric cancer.
  • To elucidate the regulatory mechanism of circ1811 in GC progression.
  • To evaluate circ1811 as a potential biomarker and therapeutic target for GC.

Main Methods:

  • Quantitative real-time PCR to assess circ1811 expression levels in GC tissues.
  • Functional assays (cell proliferation, migration, invasion, apoptosis) to determine circ1811's role in GC cells.
  • RNA immunoprecipitation (RIP) and dual-luciferase reporter assays to identify molecular interactions.

Main Results:

  • circ1811 expression was significantly downregulated in GC tissues and negatively correlated with lymphatic metastasis.
  • Overexpression of circ1811 inhibited GC cell proliferation, migration, and invasion, while promoting apoptosis.
  • circ1811 directly sponges miR-632, leading to the upregulation of DAPK1 expression.

Conclusions:

  • circ1811 functions as a tumor suppressor in gastric cancer by regulating the miR-632/DAPK1 axis.
  • circ1811 demonstrates potential as a diagnostic biomarker and therapeutic target for gastric cancer.
Keywords:
Cell proliferationCircular RNAGastric cancerMigrationceRNA

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