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Yeast Signaling01:28

Yeast Signaling

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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  1. Home
  3. An Array Of Signal-specific Moypd1 Isoforms Determines Full Virulence In The Pathogenic Fungus Magnaporthe Oryzae.
  1. Home
  3. An Array Of Signal-specific Moypd1 Isoforms Determines Full Virulence In The Pathogenic Fungus Magnaporthe Oryzae.

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An array of signal-specific MoYpd1 isoforms determines full virulence in the pathogenic fungus Magnaporthe oryzae.

Sri Bühring1, Antonia Brunner2, Klemens Heeb2

  • 1Institute of Biotechnology and Drug Research gGmbH (IBWF), Hanns-Dieter-Hüsch-Weg 17, 55128, Mainz, Germany.

Communications Biology
|March 4, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Magnaporthe oryzae produces signal-specific phosphotransfer protein MoYpd1p isoforms to manage environmental stress. These isoforms are crucial for regulating osmoregulation and orchestrating the fungus

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Fungal Genetics

Background:

  • Magnaporthe oryzae is a globally significant plant pathogen.
  • The high osmolarity glycerol (HOG) signaling pathway is vital for fungal osmoregulation.
  • The phosphotransfer protein MoYpd1p is a key component of the HOG pathway in M. oryzae.

Purpose of the Study:

  • To investigate the signal-specific production of MoYpd1p isoforms in M. oryzae.
  • To determine the role of MoYpd1p isoforms in osmoregulation and virulence.
  • To understand how different stress signals modulate MoYpd1p isoform expression.

Main Methods:

  • Analysis of transcript levels for MoYPD1 isoforms under salt (KCl) and osmotic (sorbitol) stress.
  • Observation of green fluorescent protein-fused MoYpd1p localization in response to stress.
  • Virulence assessment of M. oryzae mutant strains lacking specific MoYpd1p isoforms.

    • Main Results:

    • At least three MoYPD1 isoforms are produced in a signal-dependent manner.
    • Salt stress increases MoYPD1_T0 transcript levels, while osmotic stress elevates MoYPD1_T1 levels.
    • Mutant strains with single MoYpd1p isoforms exhibit reduced virulence, indicating a requirement for multiple isoforms.

    Conclusions:

    • MoYpd1p isoform production is specifically regulated by environmental stress signals.
    • Individual isoforms contribute to signal diversity and fine-tune the HOG pathway response.
    • Coordinated action of MoYpd1p isoforms is essential for M. oryzae virulence.