Related Experiment Video
Updated: Jul 1, 2025

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction
Published on: July 26, 2024
Characteristics of amylose-lipid complex prepared from pullulanase-treated rice and wheat flour
Sang Hoon Lee1, Wen Yan Huang1, Jinhee Hwang1
1Department of Food & Biotechnology, Korea University, Sejong, 30019 Republic of Korea.
Abstract:
This study aimed to evaluate the properties of amylose-lipid complexes in rice and wheat flours utilizing pullulanase as a debranching enzyme. Rice and flour were both treated with pullulanase before being combined with free fatty acids to form compounds denoted as RPF (rice-pullulanase-fatty acid) and FPF (flour-pullulanase-fatty acid), respectively. Our results showed that RPF and FPF had higher complex index and lower hydrolysis values than enzyme-untreated amylose-lipid complexes. Furthermore, RPF and FPF demonstrated lower swelling power and higher water solubility values, indicating changes in the physical properties of the starches. In vivo studies showed that RPF and FPF caused a smaller increase in blood glucose levels than untreated rice and flour, highlighting their potential use as functional food ingredients. These findings provide valuable information for the development of novel rice-and wheat-based foods with improved nutritional and physiological properties.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-023-01411-0.

