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Updated: Jun 23, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Fermentative optimization and characterization of exopolysaccharides from Enterococcus faecium F58 isolated from
Mariem Zanzan1,2, Youssef Ezzaky1, Fouad Achemchem1
1LASIME Research Laboratory, Bioprocess and Environment Team, Agadir Superior School of Technology, Ibn Zohr University, BP 33/S, 80150 Agadir, Morocco.
Abstract:
This study focused on optimizing the fermentation-based production of Exopolysaccharides (EPS) from Enterococcus faecium F58 initially isolated from traditional Moroccan Jben, a fresh goat cheese. Using the central composite design, yeast extract, MnSO4, and time affect EPS concentration. The highest experimental and predicted EPS production yields were 2.46 g/L ± 0.38 and 2.86 g/L, respectively. Optimal concentrations of yeast extract (4.46 g/L) and MnSO4 (0.011 g/L) were identified after 26 h at 30 °C. Characterization of EPS was conducted using SEM with EDX, XRD, and FTIR analyses. These tests revealed a specific morphology and an amorphous structure. Additionally, thermogravimetric analysis indicated adequate EPS stability up to 200 °C with anti-adhesion properties against different pathogens. This study offers valuable insights into the optimized production of EPS from Enterococcus faecium F58, which exhibits significant structural and functional properties for various applications in the food and biotechnology industries.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-023-01424-9.
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