Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mechanistic Insights into Label-Free SERS Discrimination of Structurally Similar Small-Molecule Biomarkers: A DFT Study of 4-HBA and 4-HPAA on Au Clusters.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Label-Free SERS Discrimination of Purine Small-Molecule Biomarkers: DFT Insight into Interfacial Binding Mechanism.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Performance Enhancement of SERS-Based Lateral Flow Assays: Progress and Prospective.

Analytical chemistry·2025

Same author

DNA Logic Operations-Empowered Multiple Assays for Tumor Biomarker Detecting and Tumor Cell Imaging.

Analytical chemistry·2025

Same author

DFT-Based Theoretical Study on Label-Free SERS Detection of Type B Fumonisins: New Insights into Molecular-Substrate Interactions and Quantification Strategies.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Recent Developments in SERS Microfluidic Chips: From Fundamentals to Biosensing Applications.

ACS applied materials & interfaces·2025

Same journal

Fiber and continuum scale contributions to the intrinsic and apparent fracture of soft collagenous tissue <i>via</i> cutting.

Biomaterials science·2026

Same journal

Surface morphology-regulated tissue adhesion in solid and mesoporous silica-reinforced gelatin nanocomposite hydrogels.

Biomaterials science·2026

Same journal

Nanostructured hyaluronic acid-chia mucilage film as bioactive wound dressings for accelerated skin regeneration.

Biomaterials science·2026

Same journal

Tunable bio-inspired hybrid hydrogels reprogram stem cell-derived extracellular vesicles for superior wound regeneration.

Biomaterials science·2026

Same journal

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026

Same journal

Modeling a hypoxia-integrated glioblastoma microenvironment to mimic tumor heterogeneity and chemoresistance.

Biomaterials science·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 1, 2025

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Published on: May 3, 2019

Neuroinflammatory response on a newly combinatorial cell-cell interaction chip.

Yimeng Zhao¹, Xuefei Lv¹, Yu Chen¹

¹Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China. xuefeilv@163.com.

Biomaterials Science

|March 5, 2024

Summary

This summary is machine-generated.

This study developed a microfluidic chip for modeling neuroinflammation. The chip revealed that co-cultured THP-1 cells significantly impact SH-SY5Y cell cytotoxicity and inflammatory factor release.

More Related Videos

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

On-Chip Endothelial Inflammatory Phenotyping

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

Related Experiment Videos

Last Updated: Jul 1, 2025

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Published on: May 3, 2019

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

On-Chip Endothelial Inflammatory Phenotyping

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

Area of Science:

Neuroscience
Biotechnology
Cell Biology

Background:

Neuroinflammation is a key factor in neurological disorders, involving complex intercellular interactions.
Studying these interactions is crucial but challenging with conventional methods.

Purpose of the Study:

To develop and utilize a microfluidic chip for modeling neuroinflammation and analyzing cell-cell interactions.
To investigate the influence of co-cultured THP-1 cells on SH-SY5Y cell behavior in a neuroinflammatory context.

Main Methods:

A microfluidic chip with a microarray physical barrier (15 μm spacing) was fabricated using hydrophilic surface treatment for long-term co-culture.
SH-SY5Y and THP-1 cells were co-cultured on the chip to create a neuroinflammation model.
Real-time imaging and analysis of cell morphology, viability, migration, and inflammatory factor release were performed.

Main Results:

The microfluidic model demonstrated that SH-SY5Y cytotoxicity is primarily driven by co-cultured THP-1 cells.
Complex interactions, including altered cell morphology, reduced viability, THP-1 cell migration, and inflammatory factor release, were observed.
The chip provided detailed data on cell migration patterns and inflammatory factor distribution.

Conclusions:

The developed microfluidic co-culture chip is an effective *in vitro* tool for studying neuroinflammation mechanisms.
This technology offers unique advantages for analyzing intricate cell-cell interactions and inflammatory processes in neurological disorders.