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Erythrobacter oryzae sp. nov., isolated from rice paddy soil
Hyo-Jin Lee1,2, Ji-Soo Hwang1, Eun-Kyung Lee1,2
1Institute of Microbial Ecology & Resources, Mokwon University, 88, Doanbuk-ro, Seo-gu, Daejeon, Republic of Korea.
Summary
Two novel orange-pigmented bacterial strains, Erythrobacter oryzae, were isolated from paddy soil. These aerobic, Gram-negative bacteria represent a new species within the Erythrobacter genus, expanding our understanding of soil microbial diversity.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Genomics
Background:
- The genus *Erythrobacter* comprises aerobic, Gram-negative bacteria known for their diverse metabolic capabilities and presence in various environments.
- Isolation and characterization of novel bacterial species are crucial for understanding microbial diversity and ecological roles.
Purpose of the Study:
- To isolate and characterize two novel bacterial strains from paddy soil.
- To determine the phylogenetic and taxonomic position of the novel isolates within the genus *Erythrobacter*.
- To propose a new species name for the identified strains.
Main Methods:
- Isolation of bacterial strains from paddy soil.
- Morphological, physiological, and biochemical characterization.
- Phylogenetic analysis based on 16S rRNA gene sequences.
- Genomic analysis including genome size and G+C content determination.
- Chemotaxonomic analyses (major respiratory quinone, fatty acids, polar lipids).
Main Results:
- Two novel aerobic, Gram-negative, non-motile, rod-shaped bacterial strains (COR-2T and CR-8) were isolated, forming orange-pigmented colonies.
- Phylogenetic analysis based on 16S rRNA gene sequences placed the strains within the genus *Erythrobacter*, with high similarity (99.1-99.7%) to *Erythrobacter colymbi*, *Erythrobacter donghaensis*, and *Erythrobacter tepidarius*.
- Genomic data (3,559,918 bp genome size, 67.7 mol% G+C DNA content), ANI (79.3-85.5%), and dDDH (24.1-29.1%) values, along with chemotaxonomic profiles, supported their classification as a new species.
- The strains were proposed to be named *Erythrobacter oryzae* sp. nov., with COR-2T as the type strain (KACC 22941T=JCM 35529T).
Conclusions:
- The isolated strains COR-2T and CR-8 represent a novel species within the genus *Erythrobacter*, named *Erythrobacter oryzae* sp. nov.
- The findings contribute to the taxonomic knowledge of the *Erythrobacter* genus and highlight the microbial diversity in paddy soil ecosystems.
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