An SpG-Cas9-based cytosine base editor expands the scope of genome editing in carrot plants

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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Rajesh Yarra1, Patrick J Krysan2

  • 1Department of Plant and Agroecosystem Sciences, University of Wisconsin-Madison, Madison, 53706, USA.

Plant Cell Reports
|March 5, 2024
PubMed
Abstract

Key Message:

SpG Cas9 significantly expands the genome editing scope in carrot with NGN PAM recognition.

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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