Related Experiment Video
Updated: Jul 1, 2025
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
Published on: May 19, 2014
Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells
Melissa Crawford1, Kevin Barr1, Lina Dagnino2,3
1Department of Physiology and Pharmacology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, ON, Canada.
Abstract:
In this chapter, we provide a method to purify and culture embryonic melanocytic stem cells that express green fluorescent protein in a cell-type specific manner. Isolation of melanocytic lineage cell populations that are >98% pure is accomplished through the use of GFP-based fluorescence activated cell sorting. We also provide a method to culture the purified melanoblasts and to analyze their proliferation, apoptosis, and motility properties.
