Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae
Tong Zhang1, Kun Wang2, Shengwei Dou3
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Cell Reports
|March 5, 2024
Summary
Exo84c protein is crucial for self-incompatibility in flowering plants, ensuring cross-pollination. Its absence partially breaks down this system by affecting exocyst complex regulation in the pistil.
Area of Science:
- Plant reproductive biology
- Molecular genetics
- Cell biology
Background:
- Self-incompatibility (SI) is a key mechanism in angiosperms promoting cross-pollination.
- Understanding the molecular players regulating SI is vital for crop breeding and plant reproduction.
Purpose of the Study:
- To investigate the role of Exo84c in the self-incompatibility response of Brassica napus and Arabidopsis.
- To elucidate the molecular mechanism by which Exo84c influences SI.
Main Methods:
- Gene expression analysis of Exo84c in stigma during SI.
- Generation of Exo84c knockout mutants in B. napus and SI Arabidopsis.
- Microscopic analysis of pollen-pistil interactions and exocyst complex localization.
- Assessment of protein secretion and exocyst complex recruitment.
Main Results:
- Exo84c expression is significantly upregulated in the stigma during SI.
- Disruption of Exo84c partially compromises the SI response in both species.
- Exo84c regulates exocyst complex recruitment and protein secretion inhibition during SI.
- Exo84c is involved in the turnover of exocyst-labeled compartments in papillae.
Conclusions:
- Exo84c plays a significant role in regulating the exocyst complex during the SI response.
- Exo84c likely controls the vacuolar degradation of the exocyst complex in papillae.
- This Exo84c-mediated pathway appears independent of known Brassicaceae SI pathways, contributing to SI robustness.
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