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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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SpatialView: an interactive web application for visualization of multiple samples in spatial transcriptomics

Chitrasen Mohanty1, Aman Prasad2, Lingxin Cheng1

  • 1Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI 53726, United States.

Bioinformatics (Oxford, England)
|March 6, 2024
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Summary
This summary is machine-generated.

SpatialView is a new open-source tool that visualizes spatial transcriptomics (ST) data. This interactive application helps researchers explore gene expression and cellular organization in tissues, advancing disease research.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Computational Biology

Background:

  • Spatial transcriptomics (ST) enables genome-wide gene expression analysis with spatial localization.
  • Understanding cellular heterogeneity and tissue organization is crucial in biological and clinical research.
  • Current limitations exist in interactive visualization tools for ST data, hindering comprehensive analysis.

Purpose of the Study:

  • To develop an interactive, web-based application for visualizing spatial transcriptomics data.
  • To provide a user-friendly platform for analyzing and interpreting results from ST experiments.
  • To enhance the utility of ST data for clinical and basic science investigations.

Main Methods:

  • Development of SpatialView, an open-source, web browser-based application.
  • Implementation of interactive visualization features for ST data.
  • Designed for compatibility with 10× Genomics Visium spatial transcriptomics experiments.

Main Results:

  • SpatialView offers an interactive suite for visualizing ST data and analysis results.
  • The application facilitates the exploration of spatial gene expression patterns.
  • Demonstrates a solution to the lack of interactive visualization tools for ST.

Conclusions:

  • SpatialView addresses the need for interactive visualization in spatial transcriptomics.
  • The tool is expected to benefit researchers studying disease and cellular biology.
  • Facilitates deeper insights into tissue architecture and cellular heterogeneity.