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Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing

Fu Kuroiwa1, Hiraku Suda1, Maho Yabuki1

  • 1Graduate School of Science and Engineering, Saitama University, Saitama, Japan.

Bioscience, Biotechnology, and Biochemistry
|March 6, 2024
PubMed

Keywords:
in vitro protein synthesiscell-free translationlipid monolayermembrane protein

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...