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Published on: January 28, 2020
Decreased plasma miR-140-3p is associated with coronary artery disease
Pei Mo1, Chao-Wei Tian2, Qiqi Li3
1Department of Cardiology, Guangzhou Institute of Cardiovascular Disease, Guangdong Key Laboratory of Vascular Diseases, State Key Laboratory of Respiratory Disease, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510260, China.
Insights
Reduced miR-140-3p levels are linked to coronary artery disease (CAD). Plasma miR-140-3p shows potential for evaluating CAD risk, offering a novel biomarker for disease prediction.
Area of Science:
- Biochemistry
- Genetics
- Cardiovascular Medicine
Background:
- Circulating microRNAs (c-miRNAs) show association with coronary artery disease (CAD), but lack diagnostic and predictive power.
- Novel c-miRNA discovery and validation are crucial for improving CAD risk prediction.
- Evaluating the predictive capacity of c-miRNAs is essential for clinical application.
Purpose of the Study:
- To identify novel CAD-related c-miRNAs.
- To evaluate the risk prediction capacity of identified c-miRNAs for CAD.
- To assess the clinical utility of c-miRNAs in CAD prediction.
Main Methods:
- Preliminary investigation of CAD-associated miRNAs using Applied Biosystems miRNA TaqMan® Low-Density Array (TLDA) in paired pre-CAD and CAD samples.
- Validation of candidate miRNAs in an independent case-control study (129 CAD patients, 76 controls).
- Evaluation of predictive value using machine learning (ML) algorithms and assessment of accuracy via area under the receiver operating characteristic curve (AUC).
Main Results:
- TLDA analysis revealed significantly decreased miR-140-3p in CAD stage (FC = -3.01, P = 0.007).
- miR-140-3p was significantly lower in the CAD group compared to controls (P < 0.001) and independently associated with CAD (P < 0.001).
- Inclusion of miR-140-3p improved CAD prediction accuracy in logistic regression and ML models, comparable to HDL-c and APOA1.
Conclusions:
- Reduced plasma levels of miR-140-3p are associated with CAD.
- Plasma miR-140-3p holds potential as a biomarker for evaluating CAD risk.
- miR-140-3p demonstrates practical value in CAD risk prediction, similar to established markers like HDL-c.
Background:
Although many circulating miRNAs (c-miRNAs) are associated with coronary artery disease (CAD), they are far from being the biomarker for CAD diagnosis or risk prediction. Therefore, novel c-miRNAs discovery and validation are still required, especially evaluating their prediction capacity.
Objectives:
Identify novel CAD-related c-miRNAs and evaluate its risk prediction capacity for CAD. Methods: miRNAs associated with CAD were preliminarily investigated in three paired samples representing pre-CAD stage and CAD stage of three female individuals using the Applied Biosystems miRNA TaqMan® Low-Density Array (TLDA). Then, the candidate miRNAs were further verified in an independent case-control study including 129 CAD patients and 76 controls, and their potential practical value in prediction for CAD was evaluated using a machine learning (ML) algorithm. The accuracy of classification and prediction was assessed with the area under the receiver operating characteristic curve (AUC).
Results:
TLDA analysis shows that miR-140-3p decreased significantly in CAD-stage (FC = -3.01, P = 0.007). Further study shows that miR-140-3p was significantly lower in CAD group [1.26 (0.68, 2.01)] than in control group [2.07 (1.19, 3.21)] (P < 0.001) and independently associated with CAD (P < 0.001). The addition of miR-140-3p to the variables including smoking history, HDL-c, and APOA1 improved the accuracy of classification by logistic regression and of prediction for CAD by ML models. The ML models built with miR-140-3p and HDL-c, respectively, had a similar prediction accuracy. The feature importance of miR-140-3p and HDL-c in the ML models was also similar. Decision curve analysis showed that miR-140-3p and HDL-c had almost identical net benefits.
Conclusion:
Reduced levels of miR-140-3p is linked to CAD, and it is possible to use the plasma level of miR-140-3p as a means of evaluating the risk of CAD.
