Hopanoid lipids promote soybean-Bradyrhizobium symbiosis
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Hopanoids, essential bacterial lipids, are crucial for Bradyrhizobium diazoefficiens to effectively associate with soybean plants. Their absence impairs nitrogen fixation and bacterial survival, highlighting their importance in legume symbiosis.
Area Of Science
- Microbial Ecology
- Plant-Bacterial Symbiosis
- Lipid Biochemistry
Background
- Legume-rhizobia symbioses are vital for sustainable agriculture, enhancing plant growth and soil nitrogen via nitrogen fixation.
- Hopanoids, a class of bacterial lipids, have been implicated in promoting symbioses with tropical legumes.
- Understanding hopanoid roles in agriculturally important symbiosis, like soybean and Bradyrhizobium diazoefficiens, is crucial for improving microbial inoculants.
Purpose Of The Study
- To investigate the function of hopanoids in the symbiosis between Bradyrhizobium diazoefficiens USDA110 and soybean.
- To characterize a cumate-inducible hopanoid mutant (Pcu-shc::∆shc) of B. diazoefficiens USDA110.
- To determine the impact of hopanoid deficiency on bacterial growth, stress tolerance, and symbiotic performance in planta.
Main Methods
- Utilized a cumate-inducible hopanoid mutant (Pcu-shc::∆shc) of Bradyrhizobium diazoefficiens USDA110.
- Employed Gas Chromatography-Mass Spectrometry (GC-MS) to analyze hopanoid production.
- Assessed bacterial growth under various stress conditions (osmotic, temperature, pH) and in rich medium.
- Evaluated symbiotic efficiency in soybean, including nitrogen fixation rates and bacterial survival within host tissues.
- Conducted RNA-sequencing (RNA-seq) to analyze gene expression changes in hopanoid-deficient strains.
- Performed swim plate assays to confirm motility phenotypes.
Main Results
- The Pcu-shc::∆shc strain produced no detectable hopanoids without cumate induction.
- Hopanoid deficiency impaired bacterial growth in rich medium and under osmotic, temperature, and pH stress.
- In soybean symbiosis, the mutant exhibited significantly reduced nitrogen fixation rates and poor survival within host tissues.
- RNA-seq analysis revealed that hopanoid loss downregulates genes for flagellar motility and chemotaxis while upregulating genes for nitrogen metabolism and protein secretion.
- Swim plate assays confirmed reduced motility in the absence of hopanoids.
Conclusions
- Hopanoids confer a significant fitness advantage to Bradyrhizobium diazoefficiens during symbiosis with legumes.
- Hopanoic acid deficiency impacts bacterial motility, stress tolerance, and symbiotic efficiency.
- These findings provide a basis for future research into the mechanistic roles of hopanoids in bacterial protein secretion and motility within the legume host environment.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
The collective bacteria residing in and around plant roots are termed the rhizosphere. These soil-dwelling bacterial species are incredibly diverse....
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...