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Bacterial efflux pumps excrete SYTO™ dyes and lead to false-negative staining results
Gabriel Antonio Salvador Minero1, Peter Bundgaard Larsen1, Marie Eline Hoppe1
1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus, Denmark. rikke.meyer@inano.au.dk.
The Analyst
|March 6, 2024
Abstract:
Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO™ dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO™ dyes should be used with caution on live samples.

