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Clay Sculpture-Inspired 3D Printed Microcage Module Using Bioadhesion Assembly for Specific-Shaped Tissue
Huimin Fang1, Jingyi Ju1, Lifeng Chen1
1Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 6, 2024
Summary
A new 3D bioprinting method uses modular hydrogel microcages for large tissue graft fabrication. This approach simplifies complex designs, improves cell distribution, and promotes vascularization for better graft survival.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- 3D bioprinting allows fabrication of large tissue engineering scaffolds.
- Current methods face challenges with complex designs, cell distribution, and vascularization.
Purpose of the Study:
- To develop a novel 3D bioprinting approach for constructing sizable tissue engineering grafts.
- To address limitations in cellular distribution and local angiogenesis in existing techniques.
Main Methods:
- Utilized poly (ethylene glycol) diacrylate (PEGDA)-gelatin-dopamine (PGD) hydrogel for photosensitive and adhesive microcage fabrication.
- Employed Digital Light Processing (DLP) 3D printing for fine microcage module construction.
- Developed a modular bioadhesion assembly strategy for tissue graft construction.
Main Results:
- PGD hydrogel printed microcages demonstrated flexibility for diverse shapes and cell/tissue fillings.
- In vivo experiments in nude mice showed robust vascularization and superior graft survival.
- The modular assembly strategy simplified the construction of large-volume tissue with complex components.
Conclusions:
- The novel 3D bioprinting strategy using PGD hydrogel microcages offers a promising solution for repairing diverse large tissue defects.
- This approach enhances graft survival and vascularization, simplifying the fabrication of complex tissue constructs.
