Dissolved organic nitrogen is a key to improving the biological treatment potential of landfill leachate

Biological treatment is one of the most promising efficient, low-carbon and affordable approaches for the treatment of recalcitrantly degradable wastewater, such as landfill leachate. However, even the macroscopic molecular level analysis of dissolved organic matter (DOM) is limiting to the enhancement of biological treatment efficacy, and there is an urgent need for deeper exploration of DOM to gain insights into the key constraining substances. In the present study targeting at piercing leachate organic at molecular level, nitrogen-containing dissolved organic matter (DOM_N) was identified to be the bottleneck that govern the biotreatment potential. The conclusion was made based on two series of experiments that compared the same anoxic-aerobic membrane bioreactor process (A process) operated stably at different regions, and compared with C process that coupling A process with a circulation aeration membrane bioreactor to improve aeration efficiency. The results confirmed that the relative abundance of DOM_N was absolutely dominant among the three categories of DOM in all biologically treated samples, contributing to 60.36 %-65.81 % in removed-DOM, 60.33 %-70.95 % in refractory-DOM and 63.14 %-71.36 % in derived-DOM. Specifically, the high latitude A process had much lower DOM_N removal rate than the low latitude A process (p < 0.05) and much higher refractory and derivatization rates than the low latitude A process (p < 0.05). DOM had similar results. No statistically significant differences were observed in the proportion of the three categories of DOM (DOM_N), the elements composition, and the subcategory composition of the C process compared to the A process, in which the DOM (DOM_N) derivation rate of NEC1-C (31.92 % and 33.41 %) was much higher than that of NEC1-A (20.88 % and 22.19 %). However, the AI_wa and AImod_wa of the derived-DOM (DOM_N) were significantly higher in the C process than in the A process, which implied that excessive aeration did not enhance the biological treatment potential of the A process, but instead led to the proliferation of microorganisms and the secretion of extracellular polymer substances, which resulted in the derivation of more complex compounds. The results of the correlation analysis indicated that there were some regional differences in the molecular information of DOM_N driven by climate temperature. In addition, it was worth mentioning that the nominal oxidation state of carbon (NOSC_wa) of derived-DOM_N in different regions of A process was noticeably higher than the corresponding DOM (p < 0.0001), implying that the derived-DOM_N were still highly biodegradable, in other words, there was still great room for improving the biological treatment potential of landfill leachate. The present study provided a deeper insight and analysis of landfill leachate at the molecular level (DOM_N) through multiple practical engineering cases, with a view to providing a theoretical basis for efficient optimization of biological treatment.