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Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

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Updated: Jul 1, 2025

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

A budding yeast CAGE dataset comprising two cell types.

Kei Kawakami¹, Shin-Ichi Maeda¹, Yoshiko Tanimoto¹

¹Department of Life Sciences, Shimane University School of Medicine.

Genes & Genetic Systems

|March 6, 2024

Summary

This summary is machine-generated.

This study presents a new Cap Analysis of Gene Expression (CAGE) dataset for budding yeast Saccharomyces cerevisiae a-cells and α-cells. This resource aids in understanding transcription start sites and chromatin regulation.

Keywords:

cap analysis of gene expression chromatin transcription start site yeast

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Published on: March 30, 2017

Area of Science:

Molecular Biology
Genomics
Yeast Genetics

Background:

High-resolution genome-wide analyses in Saccharomyces cerevisiae are crucial for studying chromatin regulation.
Expanding publicly available datasets enhances comparative genomics and biological insights.
Transcription start site (TSS) data at base pair resolution is vital for understanding transcription and chromatin regulation mechanisms.

Purpose of the Study:

To generate and provide a comprehensive Cap Analysis of Gene Expression (CAGE) dataset for both a-cells and α-cells of Saccharomyces cerevisiae.
To enable high-resolution analysis of transcription start sites across different yeast cell types and media conditions.
To facilitate comparative studies of transcription and chromatin regulation in yeast.

Main Methods:

Cap Analysis of Gene Expression (CAGE) was performed on a-cells and α-cells of Saccharomyces cerevisiae.
Cells were cultured in both defined and rich media to capture diverse expression profiles.
Generated CAGE data was compared with high-resolution nucleosome mapping data.

Main Results:

A novel CAGE dataset for a-cells and α-cells was successfully generated and validated for reliability.
Differential gene expression and cell type-specific genes were identified, confirming data accuracy.
Major transcription start sites were found to be located within +1 nucleosomes, approximately 30 bp from the nucleosome's promoter-proximal end.

Conclusions:

The new CAGE dataset provides valuable high-resolution TSS information for yeast a-cells and α-cells.
This resource supports research into transcription, chromatin regulation, and cell type-specific gene expression in Saccharomyces cerevisiae.
The findings highlight the positional relationship between major TSSs and the +1 nucleosome, offering insights into transcription initiation.