Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging

Area of Science:

• Biomedical Optics
• Cell Biology
• Imaging Technology

Background:

• Optical Coherence Tomography (OCT) provides cross-sectional imaging but often lacks contrast in biological tissues.
• Label-based imaging methods can be invasive and may affect cellular function.
• There is a need for non-invasive, high-contrast imaging techniques for live cell analysis.

Purpose of the Study:

• To explain the fundamental principles of Dynamic Contrast Optical Coherence Tomography (DyC-OCT) for image formation.
• To provide practical guidance on DyC-OCT system configurations for researchers.
• To review the applications of DyC-OCT in live cell imaging and functional assays.

Main Methods:

• DyC-OCT utilizes signal fluctuation patterns within standard OCT data to generate contrast.
• The review covers system configurations essential for implementing DyC-OCT.
• Applications are demonstrated through examples of cellular morphology imaging and activity monitoring.

Main Results:

• DyC-OCT enables non-invasive, label-free, volumetric imaging of live cells with enhanced contrast.
• High-quality images of cellular morphology are achievable.
• The technique is effective for monitoring dynamic changes in cellular activity and viability.

Conclusions:

• DyC-OCT is a powerful emerging imaging modality for live cell research.
• Understanding its principles and configurations is key for effective application.
• DyC-OCT facilitates advanced studies in cell biology and drug discovery through enhanced imaging capabilities.