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Related Experiment Video

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Simultaneous two-photon imaging and wireless EEG recording in mice.

Bowon Kim1,2, Weihua Ding1, Liuyue Yang1

  • 1Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA.

Heliyon
|March 7, 2024
PubMed
Summary

This study introduces CBRAIN, a wireless EEG system, to link in vivo two-photon imaging with global brain states. Researchers found a dissociation between neuronal activity and brain states during anesthesia.

Keywords:
AnesthesiaElectroencephalogram (EEG)Global brain stateIsofluraneMulti-dimensional brain activityTwo-photon

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Area of Science:

  • Neuroscience
  • Brain-Computer Interfaces
  • Anesthesiology

Background:

  • In vivo two-photon imaging offers high-resolution, longitudinal observation of neuronal activity.
  • Local neuronal activity can be influenced by global brain states like arousal and attention.
  • Integrating global brain state assessment enhances the rigor of regional neuronal activity studies.

Purpose of the Study:

  • To assess global brain state during in vivo two-photon imaging using a novel wireless EEG system.
  • To investigate the correlation between individual cell responses and concurrent EEG changes during anesthesia.
  • To explore the dissociation between cortical neuron activity and global brain activity under anesthesia.

Main Methods:

  • Developed and utilized CBRAIN (collective brain research platform aided by illuminating neural activity), a wireless EEG device, synchronized with a two-photon microscope.
  • Recorded simultaneous EEG and two-photon imaging data during isoflurane anesthesia induction.
  • Employed CBRAIN's built-in algorithms for automatic brain state detection and EEG classification.

Main Results:

  • Excitatory neuron activity in the primary somatosensory cortex ceased approximately 30 seconds after anesthesia initiation.
  • EEG exhibited transitional activity for 7 minutes, followed by a burst-suppression pattern.
  • A dissociation was observed between primary somatosensory cortex excitatory neuron activity and global brain activity under anesthesia.

Conclusions:

  • Simultaneous wireless EEG and two-photon imaging provide a practical method for correlating neuronal activity with global brain states.
  • CBRAIN facilitates automatic brain state detection, simplifying complex experimental setups.
  • This integrated approach enhances the understanding of neural dynamics in relation to global brain states.