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Quantitative Fluorescent Lateral Flow Strip Sensor for Myocardial Infarction Using Purity-Color Upconversion
1Xiamen Key Laboratory of Cardiovascular Disease, Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361005, China.
Inorganic Chemistry
|March 7, 2024
Summary
This study introduces a low-cost device for sensitive detection of cardiac biomarkers, crucial for early acute myocardial infarction diagnosis. The novel upconversion nanoparticle probes enable rapid and accurate results using a mobile phone camera.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cardiovascular Diagnostics
Background:
- Acute myocardial infarction (AMI) requires early and real-time detection for effective patient management.
- Current diagnostic methods may lack the sensitivity or cost-effectiveness needed for widespread application.
- Biomarker detection, specifically cardiac troponins, is key to diagnosing AMI.
Purpose of the Study:
- To develop a low-cost, highly sensitive device for detecting cardiac troponin T (cTnT) and cardiac troponin I (cTnI).
- To utilize dual-color upconversion nanoparticles (UCNPs) as probes for enhanced biomarker detection and integrated temperature sensing.
- To create a portable and accessible diagnostic tool for point-of-care applications.
Main Methods:
- Synthesis of dual-color UCNPs exhibiting high-purity red upconversion luminescence (UCL) and temperature-dependent multicolor emission.
- Development of a UCNPs-based lateral flow strip (LFS) for specific antigen detection.
- Integration of a low-cost laser diode, simplified laser system, and mobile-phone camera for data acquisition.
Main Results:
- UCNPs demonstrated temperature-dependent emission, with maximum sensitivity values (SR and SA) of 4.76% K-1 and 8.6% K-1 respectively.
- The UCNPs-LFS achieved a detection limit of 0.001 ng/mL for cTnI and cTnT, which is 10 times lower than the clinical cutoff.
- The device enabled specific detection of target antigens with high sensitivity.
Conclusions:
- The developed UCNPs-LFS device offers a low-cost, highly sensitive, and portable solution for cardiac biomarker detection.
- This technology has significant potential for improving early diagnosis and real-time monitoring of acute myocardial infarction.
- The integrated temperature sensing capability adds further value for diagnostic applications.
